This page lists railway museums and tourist railways in the province of Ontario, Canada.
- Agawa Canyon Tour Train (Sault Ste Marie)
- Halton County Radial Railway (Rockwood)
- Railway Museum of Eastern Ontario (Smiths Falls)
- Waterloo Central Railway (Waterloo)
- Elgin County Railway Museum (St. Thomas)
- Kenora Railway Museum (Kenora)
- Niagara Railway Museum (Fort Erie)
- Palmerston Railway Heritage Museum (Palmerston)
- Schreiber Railway Museum (Schreiber)
- Toronto Railway Museum (Toronto)
- Komoka Railway Museum (Komoka)