The CN Assiniboine subdivision runs from Canora, SK to Mutchler, SK.
| Mile | Station | Siding |
|---|---|---|
| 0.0 | CANORA Junction with CN Margo subdivision | – |
| 3.5 | FERRIER | – |
| 7.8 | AMSTERDAM | – |
| 14.1 | TADMORE | – |
| 21.2 | STURGIS JCT Junction with CN Preeceville subdivision | – |
| 23.5 | LILIAN | |
| 29.5 | LADY LAKE | 4,040 |
| 43.57 | Hotbox Detector | – |
| 44.7 | USHERVILLE | 4,400 |
| 63.1 | RESERVE | 4,090 |
| 78.1 | CLEMENCEAU | 4,130 |
| 89.6 | VARNEY | – |
| 92.2 | MUTCHLER Junction with CN Tisdale subdivision | – |
The maximum speed limit on the CN Assiniboine subdivision is 40 MPH for freight or 50 MPH for passenger trains.
The subdivision operates under the Occupancy Control System (OCS). Track between mile 0.0 and 2.0, and between 91.9 and mile 92.2 is considered subdivision track.